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pbluescript ii sk (1) vector  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript ii sk (1) vector
    Pbluescript Ii Sk (1) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+sk(1)+vector/pm24122731-104-55-60
    Average 90 stars, based on 1 article reviews
    pbluescript ii sk (1) vector - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium.
    Article Snippet: To make a constitutively expressing clone, the XbaI–HindIII fragment harboring the fcbBA genes with the RBS of the vector was transferred into the pBluescript II SK (1) vector (Stratagene) and then transformed into the E. coli TOP10 strain.

    Article Title: Preprodynorphin-expressing neurons constitute a large subgroup of somatostatin-expressing GABAergic interneurons in the mouse neocortex.
    Article Snippet: Combined labeling of immunofluorescence staining and fluorescent in situ hybridization The complementary DNA fragment corresponding to a region of glutamic acid decarboxylase 67 kDa isoform (GAD67; nucleotides 43–661 in GenBank accession no. Y12257; Tamamaki et al., 2003) or vesicular glutamate transporter 1 (VGluT1; nucleotides 855-1788 in GenBank XM_133432.2; Nakamura et al., 2007) was cloned into pBluescript II SK (1) vector (Stratagene, La Jolla, CA).

    Article Title: EVOLUTION OF INSECT METAMORPHOSIS: A MICROARRAY-BASED STUDY OF LARVAL AND ADULT GENE EXPRESSION IN THE ANT CAMPONOTUS FESTINATUS
    Article Snippet: Double-stranded cDNAs were ligated into the plasmid vector pBluescript II SK(1), which was predigested to provide compatible EcoR I ends (Stratagene).

    Article Title: A gel-less isolation of untagged plasmid DNA insert from vector backbone in homogeneous format.
    Article Snippet: Svetlana V. Kalinichenko, Mikhail V. Shepelev, Igor V. Korobko PII: S0003-2697(17)30017-9 DOI: 10.1016/j.ab.2017.01.006 Reference: YABIO 12599 To appear in: Analytical Biochemistry

    Article Title: Establishment of a Transgenic Mouse Model Specifically Expressing Human Serum Amyloid A in Adipose Tissue
    Article Snippet: A SmaI-EcoRV digested fragment containing hSAA1 was ligated into Sma1 linearized pBluescript SK II+ vector (Stratagene, La Jolla, CA) downstream of the fatty acid binding protein 4 (aP2) promoter (kindly provided by Dr. Bruce Spiegelman) .

    Article Title: Type III intermediate filament proteins interact with four-way junction DNA and facilitate its cleavage by the junction-resolving enzyme T7 endonuclease I.
    Article Snippet: The isolation from proliferating mouse and human embryo fibroblasts of SDS-stable crosslinkage products of vimentin with DNA fragments containing inverted repeats capable of cruciform formation under superhelical stress and the competitive effect of a synthetic Holliday junction on the binding of cytoplasmic intermediate filament (cIF) proteins to supercoiled DNA prompted a detailed investigation of the proteins’ capacity to associate with four-way junction DNA and to influence its processing by junction-resolving endonucleases.. Electrophoretic mobility shift analysis of reaction products obtained from vimentin and Holliday junctions under varying ionic conditions revealed efficient complex formation of the filament protein not only with the unstacked, square-planar configuration of the junctions but also with their coaxially stacked X-conformation.. Glial fibrillary acidic protein (GFAP) was less efficient and desmin virtually inactive in complex formation.

    Clone Assay:

    Article Title: Cellular expression, localization and interactions of the product of the human MOST-1 gene associated with breast and prostate cancers
    Article Snippet: .. The MOST-1 gene was cloned into a pBluescript II SK vector (Stratagene, La Jolla, CA) for expression in the T7 Quick Coupled TNT system (Promega, Madison, WI). .. In vitro TNT experiments were performed with or without Redivue L-[S35]-labeled methionine (Amersham Biosciences, Piscataway, NJ).

    Expressing:

    Article Title: Cellular expression, localization and interactions of the product of the human MOST-1 gene associated with breast and prostate cancers
    Article Snippet: .. The MOST-1 gene was cloned into a pBluescript II SK vector (Stratagene, La Jolla, CA) for expression in the T7 Quick Coupled TNT system (Promega, Madison, WI). .. In vitro TNT experiments were performed with or without Redivue L-[S35]-labeled methionine (Amersham Biosciences, Piscataway, NJ).

    Plasmid Preparation:

    Article Title: α1,2-fucosyltransferase and DNA encoding the same
    Article Snippet: Chromosomal DNA (1 μg) derived from Helicobacter mustelae ATCC43772 was completely digested with the restriction enzyme BamHI and SacI, and the digested fragment was subjected to agarose gel electrophoresis, and then a DNA fragment of about 2.4 kb was recovered using Gene Clean II Kit (available from Funakoshi). .. The plasmid vector pBluescript II SK(+) (0.1 μg, manufactured by Stratagene) was completely digested with BamHI and SacI, and then subjected to dephosporylation with alkaline phosphatase (manufactuered by Takara Shuzo). ..



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